Recombinant Human Bridging Integrator 2/BIN2 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q9UBW5
| 别称 |
Bridging Integrator 2;Breast Cancer-Associated Protein 1;BIN2;BRAP1
|
| 表达系统 |
E.coli
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| 序列 |
Met 1-Asn244
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| 蛋白编码 |
Q9UBW5
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| 种属 |
Human
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| 计算分子量 |
30.6 kDa
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| 表观分子量 |
32 kDa
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| 标签 |
N-His
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| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Bridging Integrator 2 (BIN2) is a cytoplasmic protein. BIN2 contains one BAR domain and Interacts with BIN1. BIN2 is highly expressed in some hematopoietic tissues, including peripheral blood, thymus, colon and placenta. BIN2 is an Arabidopsis GSK3-like kinase that negatively regulates brassinosteroid (BR) signaling. Genetic studies show that BIN2 is inhibited in response to BR perception at the cell surface to relieve its inhibitory effects on downstream targets.
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实验操作视频
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