Recombinant Human Carbonic Anhydrase 10/CA10 Protein (Human Cells, His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q9NS85
| 别称 |
Carbonic Anhydrase-Related Protein 10;Carbonic Anhydrase-Related Protein X;CA-RP X;CARP X;Cerebral Protein 15;CA10;CA-RPX;CARPX;HUCEP-15
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Gln22-Asn300
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| 蛋白编码 |
Q9NS85
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| 种属 |
Human
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| 计算分子量 |
32.8 kDa
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| 表观分子量 |
34 kDa
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| 标签 |
C-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Carbonic Anhydrase X (CA10) belongs to CA family of zinc metalloenzymes; which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration; renal tubular acidification and bone resorption. While CA10 is a secreted protein without Carbonic Anhydrase activity (i.e.; the reversible hydration of CO2) due to point mutations in the zinc binding site; it has esterase activity. The human and mouse CA10 are expressed in the brain; indicating that they may play a role in brain development.
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实验操作视频
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