Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag)
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- 表达系统: E.coli
- 蛋白编码: Q9Y2D0
别称 |
Carbonic Anhydrase 5B Mitochondrial;Carbonate Dehydratase VB;Carbonic Anhydrase VB;CA-VB;CA5B
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表达系统 |
E.coli
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序列 |
Cys34-Pro317
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蛋白编码 |
Q9Y2D0
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种属 |
Human
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计算分子量 |
33.8 kDa
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表观分子量 |
33 kDa
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标签 |
C-His
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
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内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.
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复溶方法 |
Not Applicable
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背景 |
Carbonic Anhydrase 5B (CA5B) is a member of alpha-carbonic anhydrase family (CAs) that catalyze the reversible hydration of carbon dioxide. CAs is associated with many biological processes; including calcification; respiration; bone resorption; acid-base balance and the formation of aqueous humor. CA5B is highly expressed in heart; pancreas; kidney; placenta; lung; and skeletal muscle; but it is restricted to the liver. CA5B is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA; CA-VA. CA5B is inhibited by coumarins; sulfonamide derivatives such as acetazolamide (AZA); saccharin; and Foscarnet.
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实验操作视频
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