Recombinant Human CDK2 Protein (E.coli, His Tag)
Price:
Size:
100μg
20μg
- 表达系统: E.coli
- 蛋白编码: P24941
别称 |
Cyclin-Dependent Kinase 2;Cell Division Protein Kinase 2;p33 Protein Kinase;CDK2;CDKN2
|
表达系统 |
E.coli
|
序列 |
Met 1-Leu298
|
蛋白编码 |
P24941
|
种属 |
Human
|
计算分子量 |
36.1 kDa
|
表观分子量 |
34 kDa
|
标签 |
N-His
|
纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% tween-80.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
|
背景 |
Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.
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实验操作视频
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