Recombinant Human CLIC1 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： O00299
| 别称 |
Chloride Intracellular Channel Protein 1;Chloride Channel ABP;Nuclear Chloride Ion Channel 27;NCC27;Regulatory Nuclear Chloride Ion Channel Protein;hRNCC;CLIC1;G6;NCC27
|
| 表达系统 |
E.coli
|
| 序列 |
Met 1-Lys241
|
| 蛋白编码 |
O00299
|
| 种属 |
Human
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| 计算分子量 |
29.0 kDa
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| 表观分子量 |
30-35 kDa
|
| 标签 |
N-His
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| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Chloride Intracellular Channel Protein 1 (CLIC1) belongs to the Chloride Channel CLIC family and contains one GST C-terminal domain. CLIC1 can be expressed in various cell types, but it is especially prominent in the heart, placenta, liver, kidney, and pancreas. CLIC1 can insert into membranes and form chloride ion channels. The channel activity depends on the pH. CLIC1 membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. CLIC1 is also involved in the regulation of the cell cycle.
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实验操作视频
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