Recombinant Human Cornulin/CRNN Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q9UBG3
| 别称 |
Cornulin;53 kDa Putative Calcium-Binding Protein;53 kDa Squamous Epithelial-Induced Stress Protein;58 kDa Heat Shock Protein;Squamous Epithelial Heat Shock Protein 53;Tumor-Related Protein;CRNN;C1orf10;DRC1;PDRC1;SEP53
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| 表达系统 |
E.coli
|
| 序列 |
Met 1-Ser140
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| 蛋白编码 |
Q9UBG3
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| 种属 |
Human
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| 计算分子量 |
17.5 kDa
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| 表观分子量 |
17 kDa
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| 标签 |
N-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Cornulin is a member of the fused gene family of molecular chaperones. Human Cornulin contains N-terminus EF-hand domains and Ca2+ binding domains, and two glutamine- and threonine-rich 60 amino acid repeats in its C-terminus. Cornulin involves in the mucosal/epithelial immune response and epidermal differentiation. Cornulin is a survival factor that participates in the clonogenicity of squamous esophageal epithelium cell lines, attenuates deoxycholic acid (DCA)-induced apoptotic cell death and release of calcium. When Cornulin is overexpressed in oral squamous carcinoma cell lines, it regulates negatively cell proliferation by the induction of G1 arrest.
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实验操作视频
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