Recombinant Human Creatine Kinase BB/CKB Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P12277
| 别称 |
B-CK;CKB;Creatine Kinase BB
|
| 表达系统 |
E.coli
|
| 序列 |
Met1-Lys381
|
| 蛋白编码 |
P12277
|
| 种属 |
Human
|
| 计算分子量 |
44.8 kDa
|
| 表观分子量 |
50 kDa
|
| 标签 |
N-His
|
| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Creatine kinase B-type (CKB) belongs to the ATP:guanido phosphotransferase family. It has dimer of identical or non-identical chains with MM being the major form in skeletal muscle and myocardium. MB exists in myocardium, and BB exists in many tissues, especially brain. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure.
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实验操作视频
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