Recombinant Human Cytochrome C/CYCS Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P99999
| 别称 |
Cytochrome C;CYCS;CYC
|
| 表达系统 |
E.coli
|
| 序列 |
Gly2-Glu105
|
| 蛋白编码 |
P99999
|
| 种属 |
Human
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| 计算分子量 |
12.8 kDa
|
| 表观分子量 |
16 kDa
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| 标签 |
C-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 200mM NaCl, 50% Glycerol, 0.05% Tween 80, pH7.0.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Cytochrome C (CYCS) is a small heme protein that belongs to the cytochrome c family. It is found loosely associated with the inner membrane of the mitochondrion. Cytochrome C is a highly soluble protein that functions as a central component of the electron transport chain in mitochondria. CYCS transfers electrons between Complexes III (Coenzyme Q - Cyt C reductase) and IV (Cyt C oxidase). CYCS plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of Cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
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实验操作视频
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