Recombinant Human Dermatopontin/DPT Protein (Fc & His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q07507
| 别称 |
Dermatopontin;Tyrosine-rich acidic matrix protein;TRAMP and DPT;
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| 表达系统 |
HEK293 Cells
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| 序列 |
Gln19-Val201
|
| 蛋白编码 |
Q07507
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| 种属 |
Human
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| 计算分子量 |
49.9 kDa
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| 表观分子量 |
50-60 kDa
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| 标签 |
C-Fc-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 4% Sucrose, 4% mannitol, 0.02% Tween80, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Dermatopontin, also known as Tyrosine-rich acidic matrix protein, TRAMP and DPT, is a secreted protein which belongs to the dermatopontin family. DPT is expressed in various tissues, such as fibroblasts, heart, skeletal muscle, brain and pancreas. It seems to mediate adhesion by cell surface integrin binding. DPT may serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. DPT can enhance TGFB1 activity through interaction with decorin. In addition, DPT accelerates collagen fibril formation, stabilizes collagen fibrils against low-temperature dissociation and inhibits cell proliferation.
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实验操作视频
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