Recombinant Human ECH1 Protein
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- 表达系统： E.coli
- 蛋白编码： Q13011
| 别称 |
Delta(3;5)-Delta(2;4)-dienoyl-CoA isomerase;mitochondrial;ECH1;
|
| 表达系统 |
E.coli
|
| 序列 |
Thr34-Leu328
|
| 蛋白编码 |
Q13011
|
| 种属 |
Human
|
| 计算分子量 |
34.5 kDa
|
| 表观分子量 |
30-35 kDa
|
| 标签 |
N-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 5mM EDTA, pH 8.0.
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| 复溶方法 |
Not Applicable
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| 背景 |
Human delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase(ECH1) is a member of the hydratase/isomerase superfamily and contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The rat orthologlocalizes to the matrix of both the peroxisome and mitochondria.It can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 plays an important role in the auxiliary step of the fatty acid beta-oxidation pathway.
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实验操作视频
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