Recombinant Human FGF-7/KGF Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P21781
- 应用性： Cell culture
| 别称 |
Fibroblast growth factor 7;FGF-7;Heparin-binding growth factor 7;HBGF-7;Keratinocyte growth factor;FGF7
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| 表达系统 |
HEK293 Cells
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| 序列 |
Cys32-Thr194
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| 蛋白编码 |
P21781
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| 种属 |
Human
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| 计算分子量 |
20.0 kDa
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| 表观分子量 |
20-27 kDa
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| 标签 |
C-His
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| 生物活性 |
Measured in a cell proliferation assay using HaCaT cells. The ED50 for this effect is 10.94 ng/ml.
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| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities; and are involved in a variety of biological processes; including embryonic development; cell growth; morphogenesis; tissue repair; tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor; whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.
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实验操作视频
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