Recombinant Human GDF5/BMP-14 Protein
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- 表达系统： E.coli
- 蛋白编码： P43026
- 应用性： Cell culture
| 别称 |
Growth/differentiation factor 5;GDF-5;Bone morphogenetic protein 14;BMP-14;Cartilage-derived morphogenetic protein 1;CDMP-1;Lipopolysaccharide-associated protein 4;LAP-4;LPS-associated protein 4;Radotermin;CDMP1
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| 表达系统 |
E.coli
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| 序列 |
Ala382-Arg501
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| 蛋白编码 |
P43026
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| 种属 |
Human
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| 计算分子量 |
14.5 kDa
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| 表观分子量 |
18 kDa
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| 标签 |
C-His
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| 生物活性 |
Measure by its ability to induce alkaline phosphatase production by ATDC5 cells.The ED50 for this effect is <14 ng/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Growth Differentiation Factor 5(GDF-5, BMP-14) is a member of the BMP family of TGFβ superfamily proteins. Human GDF-5, -6, and -7 are a defined subgroup of the BMP family. GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) Nterminal proregion and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-IIor Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis. Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis.
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实验操作视频
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