Recombinant Human GSS Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P48637
| 别称 |
Glutathione Synthetase;GSH Synthetase;GSH-S;Glutathione Synthase;GSS
|
| 表达系统 |
E.coli
|
| 序列 |
Ala2-Val474
|
| 蛋白编码 |
P48637
|
| 种属 |
Human
|
| 计算分子量 |
53.5 kDa
|
| 表观分子量 |
55 kDa
|
| 标签 |
C-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Glutathione Synthetase belongs to the eukaryotic GSH synthase family. Glutathione Synthetase is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine to form glutathione. Glutathione play an important role in a variety of biological functions, including detoxification of xenobiotics, protection of cells from oxidative damage by free radicals, and membrane transport. The protein functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in Glutathione Synthetase can also cause the glutathione synthetase deficiency of erythrocytes, which is a mild form causing hemolytic anemia.
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实验操作视频
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