Recombinant Human IL-23 alpha & Mouse IL-12 beta Heterodimer (C-6His)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q9NPF7&P43432
- 应用性： Cell culture
| 别称 |
SGRF;IL-23p19;CLMF p40;IL-12 subunit p40;NKSF2
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| 表达系统 |
HEK293 Cells
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| 序列 |
Arg20-Pro189&Met23-Ser335
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| 蛋白编码 |
Q9NPF7&P43432
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| 种属 |
Human & Mouse
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| 计算分子量 |
56.9 kDa
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| 表观分子量 |
65-80 kDa
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| 标签 |
C-His
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| 生物活性 |
Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 357.68 ng/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
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实验操作视频
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