Recombinant Human KI67 protein(His tag)
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- 表达系统： E.coli
- 蛋白编码： P46013
| 别称 |
Antigen identified by monoclonal antibody Ki-67;Antigen KI-67;Antigen Ki67
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| 表达系统 |
E.coli
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| 序列 |
Lys1001-Pro1112
|
| 蛋白编码 |
P46013
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| 种属 |
Human
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| 计算分子量 |
12.4 kDa
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| 表观分子量 |
13 kDa
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| 标签 |
N-His
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| 纯度 |
> 85 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
Please contact us for more information.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly.Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface.Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.Binds DNA, with a preference for supercoiled DNA and AT-rich DNA.Does not contribute to the internal structure of mitotic chromosomes.May play a role in chromatin organization.It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
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实验操作视频
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