Recombinant Human MIF Protein
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- 表达系统： E.coli
- 蛋白编码： P14174
| 别称 |
Macrophage migration inhibitory factor;MIF;MMIF;Glycosylation-inhibiting factor;GLIF;L-dopachrome tautomerase;Phenylpyruvate tautomerase
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| 表达系统 |
E.coli
|
| 序列 |
Met 1-Ala115
|
| 蛋白编码 |
P14174
|
| 种属 |
Human
|
| 计算分子量 |
13.3 kDa
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| 表观分子量 |
15 kDa
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| 标签 |
C-His
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without asignal sequence .Secretion occurs nonclassically via an ABCA1 transporter.Pro-inflammatory cytokine.Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites ofinflammation suggests a role as mediator in regulating the function of acrophages in host defense.Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase anddopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clearwhether the tautomerase activity has any physiological relevance, and whether it is important for cytokineactivity.
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实验操作视频
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