Recombinant Human/Mouse FGF-8b/FGF8B Protein
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- 表达系统： E.coli
- 蛋白编码： P55075-3/P37237-2
- 应用性： Cell culture
| 别称 |
Fibroblast growth factor 8;Androgen-induced growth factor;Heparin-binding growth factor 8;AIGF;HBGF-8;FGF-8B
|
| 表达系统 |
E.coli
|
| 序列 |
Gln23-Arg215
|
| 蛋白编码 |
P55075-3/P37237-2
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| 种属 |
Human/Mouse
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| 计算分子量 |
22.5 kDa
|
| 表观分子量 |
23 kDa
|
| 标签 |
None
|
| 生物活性 |
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 21.87 ng/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB,300mM NaCl,2% Sucrose,0.02% Tween 80,pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Fibroblast growth factor 8 (FGF-8) is a member of the fibroblast growth factor family. It is discovered as a growth factor essential for the androgen--dependent growth of mouse mammary carcinoma cells. Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial--mesenchymal transitions. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. It is required for normal brain, eye, ear, limb development during embryogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.
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实验操作视频
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