Recombinant Human/Mouse/Rat Activin A protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P08476
- 应用性： Cell culture
| 别称 |
Inhibin beta A chain;INHBA;Activin A
|
| 表达系统 |
E.coli
|
| 序列 |
Gly 311-Ser 426
|
| 蛋白编码 |
P08476
|
| 种属 |
Human/Mouse/Rat
|
| 计算分子量 |
13.1 kDa
|
| 表观分子量 |
15 kDa
|
| 标签 |
None
|
| 生物活性 |
Measure by its ability to inhibit the proliferation of mouse MPC-11 cells. The ED50 for this effect is <10 ng/mL. The specific activity of recombinant human Activin A is approximately >1.0 x 103 IU/mg.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits.
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实验操作视频
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