Recombinant Human NANOG protein (His tag)
Price:
Size:
100μg
20μg
- 表达系统: E.coli
- 蛋白编码: Q9H9S0
别称 |
Embryonic stem cell specific homeobox protein;ENK;FLJ12581;hNanog;Homeobox protein NANOG
|
表达系统 |
E.coli
|
序列 |
Lys 94-Ser 162
|
蛋白编码 |
Q9H9S0
|
种属 |
Human
|
计算分子量 |
7.5 kDa
|
表观分子量 |
12 kDa
|
标签 |
N-His
|
纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
内毒素 |
Please contact us for more information.
|
保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
|
背景 |
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
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实验操作视频
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