Recombinant Human Nucleobindin-2/NUCB2 Protein
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- 表达系统： E.coli
- 蛋白编码： P80303
| 别称 |
Nucleobindin-2;DNA-binding protein NEFA;Gastric cancer antigen Zg4;Prepronesfatin;Nesfatin-1;NUCB2;NEFA
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| 表达系统 |
E.coli
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| 序列 |
Val25-Leu106
|
| 蛋白编码 |
P80303
|
| 种属 |
Human
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| 计算分子量 |
9.6 kDa
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| 表观分子量 |
10 kDa
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| 标签 |
None
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 10mM Sodium Phosphate,pH6.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2). Nesfatin-1 is a neuropeptide produced in the hypothalamus of mammals. It participates in the regulation of hunger and fat storage. Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets β-cells, gastric endocrine cells and adipocytes. Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner. Nesfatin-1 may also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.
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实验操作视频
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