Recombinant Human PDGF-BB Protein
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- 表达系统： E.coli
- 蛋白编码： P01127
- 应用性： Cell culture
| 别称 |
Platelet-Derived Growth Factor Subunit B;PDGF Subunit B;PDGF-2;Platelet-Derived Growth Factor B Chain;Platelet-Derived Growth Factor Beta Polypeptide;Proto-Oncogene c-Sis;Becaplermin;PDGFB;PDGF2;SIS
|
| 表达系统 |
E.coli
|
| 序列 |
Ser82-Thr190
|
| 蛋白编码 |
P01127
|
| 种属 |
Human
|
| 计算分子量 |
12.4 kDa
|
| 表观分子量 |
14 kDa
|
| 标签 |
None
|
| 生物活性 |
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 5-20 ng/ml.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.2 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
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实验操作视频
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