Recombinant Human PDILT Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： Q8N807
| 别称 |
Protein Disulfide-Isomerase-Like Protein of the Testis;PDILT
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Ser21-Leu584
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| 蛋白编码 |
Q8N807
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| 种属 |
Human
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| 计算分子量 |
65.5 kDa
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| 表观分子量 |
92 kDa
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| 标签 |
C-His
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| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
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| 复溶方法 |
Not Applicable
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| 背景 |
Protein Disulfide-Isomerase-Like Protein of the Testis (PDILT) is a protein that belongs to the protein disulfide isomerase family. Human PDILT is synthesized as a 584 amino acid precursor that contains an 20 amino acid signal sequence and a 564 amino acid mature chain. PDILT contains 1 thioredoxin domain lacks the conserved redox-active Cys at position 417 which is replaced by a Ser residue, suggesting that it lacks thioredoxin activity. PDILT is an enzyme in the endoplasmic reticulum in eukaryotes. It is not a disulfide-linked homodimer. The PDILT protein can interacts with ERO1L and CLGN. PDILT probable redox-inactive chaperone involved in spermatogenesis.
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实验操作视频
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