Recombinant Human PKLR Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P30613
| 别称 |
Pyruvate Kinase Isozymes R/L;Pyruvate Kinase 1;R-Type/L-Type Pyruvate Kinase;Red Cell/Liver Pyruvate Kinase;PKLR;PK1;PKL
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Met 1-Ser574
|
| 蛋白编码 |
P30613
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| 种属 |
Human
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| 计算分子量 |
62.9 kDa
|
| 表观分子量 |
58 kDa
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| 标签 |
C-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 5% Trehalose, 5% Mannitol, 0.02% Tween 80, 50% Glycerol, 1mM EDTA, 1mM DTT, pH8.0.
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| 复溶方法 |
Not Applicable
|
| 背景 |
Pyruvate Kinase Isozymes R/L (PKLR) belongs to the pyruvate kinase family, There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver; R is found in red cells; M1 is the main form in muscle, heart and brain; M2 is found in early fetal tissues. PKLR exists as a homotetramer and catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in PKLR are also the cause of pyruvate kinase deficiency of red cells, which is a frequent cause of hereditary non-spherocytic hemolytic anemia.
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实验操作视频
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