Recombinant Human SCF/c-Kit Ligand Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P21583
- 应用性： Cell culture
| 别称 |
Kit Ligand;Mast Cell Growth Factor;MGF;Stem Cell Factor;SCF;c-Kit ligand;KITLG;MGF;SCF
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Glu26-His214
|
| 蛋白编码 |
P21583
|
| 种属 |
Human
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| 计算分子量 |
22.0 kDa
|
| 表观分子量 |
30-50 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-10 ng/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid; erythroid; and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
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实验操作视频
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