Recombinant Human Semaphorin 3A/SEMA3A Protein (His & Flag Tag)
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- 表达系统: HEK293 Cells
- 蛋白编码: Q14563
别称 |
Semaphorin III;SEMA3A;SEMAD;Hsema-I;SEMA1;SemD;Semad;coll-1;Hsema-III;Sema III
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表达系统 |
HEK293 Cells
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序列 |
Lys26-Phe546
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蛋白编码 |
Q14563
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种属 |
Human
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计算分子量 |
61.3 kDa
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表观分子量 |
70-75 kDa
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标签 |
N-His-Flag
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
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内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH 8.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
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背景 |
emaphorin-3A is a secreted protein which belongs to the semaphorin family. Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain.This protein can function as either a chemorepulsive agent; inhibiting axonal outgrowth; or as a chemoattractive agent; stimulating the growth of apical dendrites. In both cases; the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines.
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实验操作视频
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