Recombinant Human TFIIB/GTF2B Protein (GST Tag)
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- 表达系统： E.coli
- 蛋白编码： Q00403
| 别称 |
Transcription Initiation Factor IIB;General Transcription Factor TFIIB;S300-II;GTF2B;TF2B;TFIIB
|
| 表达系统 |
E.coli
|
| 序列 |
Met 1-Leu316
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| 蛋白编码 |
Q00403
|
| 种属 |
Human
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| 计算分子量 |
61.6 kDa
|
| 表观分子量 |
62 kDa
|
| 标签 |
N-GST
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| 纯度 |
> 80 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.
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实验操作视频
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