Recombinant Human TGF beta 3 protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P10600
- 应用性： Cell culture
| 别称 |
ARVD;ARVD1;LDS5;RNHF;TGFB3;TGF-B3
|
| 表达系统 |
E.coli
|
| 序列 |
Ala 301-Ser 412
|
| 蛋白编码 |
P10600
|
| 种属 |
Human
|
| 计算分子量 |
13.7 kDa
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| 表观分子量 |
13 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED50 for this effect is <50 pg/mL. The specific activity of recombinant human TGF beta 3 is > 2 x 107 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
TGF?-beta 3 (transforming growth factor-beta 3) is a member of a TGF?-beta superfamily subgroup that is defined by their structural and functional similarities. TGF-beta 3 and its closely related proteins, TGF-beta 1 and ? beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial-?mesenchymal transition. The non-redundant biological effects of TGF-? beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development. Rat TGF?-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 23 aa signal peptide and a 389 aa proprotein. TGF-beta 3 is secreted as a latent complex. This latent form of TGF-beta 3 is activated by integrins, thrombospondin-1, plasmin, and matrix metalloproteases. It can also be activated by extreme pH and reactive oxygen species. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-? beta RI or ALK-?1, to modulate transcription through Smad phosphorylation. The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF-? beta RIII and endoglin) and the subsequent initiation of Smad--dependent or -independent signaling pathways.
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实验操作视频
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