Recombinant Human UBE2D4 Protein (GST Tag)
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- 表达系统： E.coli
- 蛋白编码： Q9Y2X8
| 别称 |
Ubiquitin-Conjugating Enzyme E2 D4;HBUCE1;Ubiquitin Carrier Protein D4;Ubiquitin-Protein Ligase D4;UBE2D4;UBCH5D
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| 表达系统 |
E.coli
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| 序列 |
Met 1-Met 147
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| 蛋白编码 |
Q9Y2X8
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| 种属 |
Human
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| 计算分子量 |
43.5 kDa
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| 表观分子量 |
40 kDa
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| 标签 |
N-GST
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
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| 复溶方法 |
Not Applicable
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| 背景 |
Ubiquitin-Conjugating Enzyme E2 D4 (UBE2D4) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2D4 has been proposed to participate in Ubl conjugation pathway. UBE2D4 takes part in post-translational protein modification; protein K6-linked ubiquitination; protein K11-linked ubiquitination; protein K27-linked ubiquitination; protein K29-linked ubiquitination; protein K48-linked ubiquitination; and protein K63-linked ubiquitination. UBE2D4 regulate of protein metabolic process. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro; UBE2D4 able to promote polyubiquitination using all 7 ubiquitin Lys residues; but may prefer 'Lys-11' and 'Lys-48'-linked poly-ubiquitination.
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实验操作视频
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