Recombinant Human Wnt3a/Wnt-3a
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- 表达系统： HEK293 Cells
- 应用性： Cell culture
| 别称 |
MGC119418;MGC119419;MGC119420;protein Wnt-3a;wingless-type MMTV integration site family;member 3A;Wnt3a;Wnt-3a
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| 表达系统 |
HEK293 Cells
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| 种属 |
Human
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| 计算分子量 |
58.5 kDa
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| 表观分子量 |
66-73 kDa
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| 标签 |
None
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| 生物活性 |
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 71.45 ng/ml.
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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| 制剂 |
Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 500mM NaCl, pH8.2.
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| 复溶方法 |
Not Applicable
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| 背景 |
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
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实验操作视频
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