Recombinant Mouse bFGF/FGF2 Protein (His Tag)
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- 表达系统: E.coli
- 蛋白编码: P15655
- 应用性: Cell culture
别称 |
bFGF;Fgf-2;Fgfb
|
表达系统 |
E.coli
|
序列 |
Ala11-Ser154
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蛋白编码 |
P15655
|
种属 |
Mouse
|
计算分子量 |
17.2 kDa
|
表观分子量 |
19 kDa
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标签 |
N-His
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生物活性 |
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <1.5 ng/mL. The specific activity of recombinant mouse FGF-2 is approximately >1 x 106 IU/mg.
|
纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from sterile PBS with 0.01% sarkosyl,pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
|
背景 |
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies. bFGF/FGF2 Protein & Antibody Products
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实验操作视频
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