Recombinant Mouse Complement Component C3a/C3a Protein
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- 表达系统： E.coli
- 蛋白编码： P01027
| 别称 |
Complement Component C3a;Anaphylatoxin;C3a
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| 表达系统 |
E.coli
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| 序列 |
Ser671-Arg748
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| 蛋白编码 |
P01027
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| 种属 |
Mouse
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| 计算分子量 |
9.2 kDa
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| 表观分子量 |
9 kDa
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| 标签 |
None
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Complement is defined as key part of innate immunity and as the first line of defense in the fight against invading pathogens. Complement 3 (C3) is the most abundant component of the complement cascade and the convergent point for all three major complement activation pathways: namely classical, alternative and mannose-binding lectin pathways. Complement activation leads to the formation of the C3 convertase, which cleaves C3 into the key effector molecules, C3a (anaphylatoxin) and C3b (opsonin) which then drive microbe removal. By binding to C3a receptor (C3aR), C3a exhibits potent anaphylatoxin activity, including increased vascular permeability, triggering degranulation of mast cells, inflammation, and activating leukocytes.
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实验操作视频
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