Recombinant Mouse FAP protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P97321
| 别称 |
170 kDa melanoma membrane bound gelatinase;170 kDa melanoma membrane-bound gelatinase;DPPIV;FAP;FAPA;Fibroblast activation protein alpha;Integral membrane serine protease;SEPR;Seprase;FAPalpha;SIMP
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| 表达系统 |
E.coli
|
| 序列 |
Leu26-Gly349
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| 蛋白编码 |
P97321
|
| 种属 |
Mouse
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| 计算分子量 |
38 kDa
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| 表观分子量 |
35-40 kDa
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| 标签 |
N-His & C-His
|
| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
Please contact us for more information.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein. Have also dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB).
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实验操作视频
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