Recombinant Mouse IL-17B protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q9QXT6
- 应用性： Cell culture
| 别称 |
TNFSF1B;Lymphotoxin-alpha (LT-α);LTalpha;Ltx;TNF-be;TNFSF1;Tnf;Tnfb;Tnfsf;Tnfsf1b;Tnlg1e;hlb38;hlb382;lymph
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| 表达系统 |
E.coli
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| 序列 |
His 21-Phe 180
|
| 蛋白编码 |
Q9QXT6
|
| 种属 |
Mouse
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| 计算分子量 |
19.0 kDa
|
| 表观分子量 |
17-25 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measure by its ability to induce IL-8 secretion in HepG2 cells. The ED50 for this effect is <1.5 ng/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin-17B (IL-17B) is a member of IL-17 cytokines family that plays important roles in host defence responses and inflammatory diseases. In addition to IL-17B, members of the IL-17 family include IL-17A, IL-17C, IL-17D, IL-17E and IL-17F. The six IL-17 cytokines are highly conserved at C terminus, and contain five spatially conserved cysteine residues that mediate dimerization. Expression of IL-17B protein has been reported in neurons, testis, ovary, stomach, pancreas, small intestine and chondrocytes. IL-17B binds to Interleukine-17 receptor B (IL-17 RB) and induces the production of inflammatory cytokines and the infiltration of inflammatory immune cells.
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实验操作视频
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