Recombinant Mouse IL-9 protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P15247
- 应用性： Cell culture
| 别称 |
Interleukin-12 subunit beta;IL-12 subunit p40;IL-12B;Cytotoxic Lymphocyte Maturation Factor 40 kDa subunit (CLMF p40);NK cell Stimulating Factor Chain 2
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| 表达系统 |
E.coli
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| 序列 |
Gln 19-Pro 144
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| 蛋白编码 |
P15247
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| 种属 |
Mouse
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| 计算分子量 |
15.0 kDa
|
| 表观分子量 |
12 kDa
|
| 标签 |
N-His
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| 生物活性 |
Measure by its ability to induce proliferation in MO7e cells.The ED50 for this effect is <0.2 ng/mL.The specific activity of recombinant mouse IL-9 is > 5 x 106 IU/mg.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin-9 (IL-9) is a secreted protein that belongs to the IL-7/IL-9 family.Mature mouse IL-9 shares 57% and 74% amino acid sequence identity with human and rat IL-9, respectively. IL-9 supports IL-2 independent and IL-4 independent growth of helper T-cells. IL-9 stimulates cell proliferation and prevents apoptosis. It functions through the IL-9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. IL-9 has been identified as a candidate gene for asthma. IL-9 is a determining factor in the pathogenesis of bronchial hyperresponsiveness.
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实验操作视频
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