Recombinant Mouse Interleukin-7/IL-7 Protein (His Tag)
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- 表达系统： HEK293 Cells
- 蛋白编码： P10168
- 应用性： Cell culture
| 别称 |
IL-7;IL-7 interleukin-7;interleukin-7;Lymphopoietin -1;PBGF
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Glu26-Ile154
|
| 蛋白编码 |
P10168
|
| 种属 |
Mouse
|
| 计算分子量 |
15.9 kDa
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| 表观分子量 |
22-28 kDa
|
| 标签 |
C-His
|
| 生物活性 |
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL).The ED50 for this effect is 60-1000 pg/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Mouse interleukin-7(IL-7) is the member of hemopoietin family which is important to the differentiation, proliferation, and survival of lymphocyte. Mouse IL-7 shares approximately 88% aa sequence identity with rat IL-7 and 58-60% with human, equine, bovine, ovine, porcine, feline and canine IL-7. It is widely expressed in primary and secondary lymphoid tissues cell and stromal epithelial cells of the thymus, bone marrow, and intestines. IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development. It is important for proliferation during certain stages of B-cell maturation. IL-7 contributes to the maintenance of all na?ve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2. It is required for optimal T cell-dendritic cell interaction.
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实验操作视频
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