Recombinant Mouse Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)
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- 表达系统： HEK293 Cells
- 蛋白编码： A7E1W8
- 应用性： BLI
| 别称 |
Angiopoietin-related protein 4;425O18-1;Angiopoietin-like protein 4;Fasting-induced adipose factor;Hepatic fibrinogen/angiopoietin-related protein;HFARP;Secreted protein Bk89;Angptl4;Farp;Fiaf;Ng27
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| 表达系统 |
HEK293 Cells
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| 序列 |
Arg24-Thr262
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| 蛋白编码 |
A7E1W8
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| 种属 |
Mouse
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| 计算分子量 |
26.5 kDa
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| 表观分子量 |
30-40 kDa
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| 标签 |
C-His
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| 生物活性 |
Loaded Anti-Human Siglec15 mAb-mFc on AMQ Biosensor, can bind Mouse Siglec-15-His with an affinity constant of 0.33 nM as determined in BLI assay.
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
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| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, 150mM NaCl, 5% Thehalose, 0.3% Chaps, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO?-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts
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实验操作视频
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