Recombinant Mouse TNF-alpha/TNFA Protein
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- 表达系统： E.coli
- 蛋白编码： P06804
- 应用性： Cell culture
| 别称 |
Tumor Necrosis Factor;Cachectin;TNF-Alpha;Tumor Necrosis Factor Ligand Superfamily Member 2;TNF-a;Tumor Necrosis Factor;Membrane Form;Tumor Necrosis Factor;Tnf;Tnfsf2;DIF;TNF-alpha;Tnfa;TNFalpha;Tnfsf1a;TNFSF2
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| 表达系统 |
E.coli
|
| 序列 |
Asp89-Leu235
|
| 蛋白编码 |
P06804
|
| 种属 |
Mouse
|
| 计算分子量 |
16.4 kDa
|
| 表观分子量 |
14 kDa
|
| 标签 |
None
|
| 生物活性 |
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2-8 pg/ml.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.01 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.
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实验操作视频
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