Recombinant Mouse TNF-alpha/TNFA Protein (aa 80-235)
Price:
- 表达系统: E.coli
- 蛋白编码: P06804
- 应用性: Cell culture
别称 |
Tumor Necrosis Factor;Cachectin;TNF-Alpha;Tumor Necrosis Factor Ligand Superfamily Member 2;TNF-a;Tumor Necrosis Factor;Membrane Form;Tumor Necrosis Factor;Tnf;Tnfsf2;DIF;TNF-alpha;Tnfa;TNFalpha;Tnfsf1a;TNFSF2
|
表达系统 |
E.coli
|
序列 |
Leu 80-Leu 235
|
蛋白编码 |
P06804
|
种属 |
Mouse
|
计算分子量 |
18.2 kDa
|
表观分子量 |
16 kDa
|
标签 |
C-His
|
生物活性 |
Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately >2.5x 107 IU/mg.
|
纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
|
背景 |
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. TNF-alpha Protein & Antibody
|
实验操作视频
新品推荐
Recombinant Human IL-6 Protein (Fc Tag)
Recombinant Mouse BCR protein (His tag)
Recombinant Mouse IL6 protein(His tag)
Recombinant Rat IL2/IL-2/Interleukin-2 protein (His tag)
Recombinant SARS-CoV-2 Nucleocapsid Protein (His Tag)
Recombinant Human Fyn protein(His tag)
Recombinant Mouse CD96/TACTILE protein (His tag)