Recombinant Rat AST protein (His tag)
Price:
- 表达系统： HEK293 Cells
- 蛋白编码： P13221
| 别称 |
Aspartate aminotransferase;cytoplasmic;cAspAT;Cysteine aminotransferase;cytoplasmic;Cysteine transaminase;cytoplasmic;cCAT;Glutamate oxaloacetate transaminase 1;Transaminase A;Got1
|
| 表达系统 |
HEK293 Cells
|
| 序列 |
Met1-Gln413
|
| 蛋白编码 |
P13221
|
| 种属 |
Rat
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| 计算分子量 |
45.3 kDa
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| 表观分子量 |
45 kDa
|
| 标签 |
C-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
Please contact us for more information.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
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实验操作视频
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