Recombinant Rhesus macaque IL-33/Interleukin-33 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： F7DLG4
| 别称 |
Interleukin-33;IL-33;Interleukin-1 Family Member 11;IL-1F11;Nuclear Factor From High Endothelial Venules;NF-HEV;IL33;C9orf26;IL1F11;NFHEV
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| 表达系统 |
E.coli
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| 序列 |
Ser112-Ile270
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| 蛋白编码 |
F7DLG4
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| 种属 |
Rhesus macaque
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| 计算分子量 |
20.3 kDa
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| 表观分子量 |
21 kDa
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| 标签 |
N-His
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| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Interleukin-33 (IL-33) belongs to the IL-1 superfamily. IL33 is highly expressed in endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes, but almost undetectable in placenta. IL33 induces the production of T helper-2 (Th2)-associated cytokines. IL-33 is a cytokine that mediates its biological effects by binding to and signals through IL1RL1/ST2 and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines from polarized Th2 cells.
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实验操作视频
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