Recombinant Swine FGF-2 protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： NP_001392443
- 应用性： Cell culture
| 别称 |
Fgfb;bFGF;FGF-basic
|
| 表达系统 |
E.coli
|
| 序列 |
Ala 2-Ser 155
|
| 蛋白编码 |
NP_001392443
|
| 种属 |
Porcine
|
| 计算分子量 |
18.1 kDa
|
| 表观分子量 |
17 kDa
|
| 标签 |
N-His
|
| 生物活性 |
Measure by its ability to induce proliferation in 3T3 cells. The ED50 for this effect is <2 ng/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS with 0.01% sarkosyl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Fibroblast growth factor 2(FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial; tumor and other cell types. It involved in developmental processes and regulates differentiation; proliferation; and migration; FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase.
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实验操作视频
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