Recombinant Swine TNF alpha protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P23563
- 应用性： Cell culture
| 别称 |
TNFSF2;Cachectin;Differentiation-inducing factor (DIF);Necrosin;Cytotoxin;TNS?F1A
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| 表达系统 |
E.coli
|
| 序列 |
Leu 77-Leu 232
|
| 蛋白编码 |
P23563
|
| 种属 |
Porcine
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| 计算分子量 |
18.1 kDa
|
| 表观分子量 |
19 kDa
|
| 标签 |
C-His
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| 生物活性 |
Measure by its ability to induce cytotoxicity in PK15 cells in the presence of the actinomycin D.The ED50 for this effect is <15 pg/mL.
|
| 纯度 |
> 98 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
Tumor necrosis factor alpha (TNFα) is the prototypic ligand of the TNF superfamily. TNFα forms a homotrimer and functions by activating two types of receptors TNF-R1 (TNF receptor type 1,p55R) and TNF-R2 (TNF receptor type 2,p75R). TNFα is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
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实验操作视频
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