Recombinant Thermobifida fusca Cutinase Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： E5BBQ3
| 别称 |
Cutinase
|
| 表达系统 |
E.coli
|
| 序列 |
Ala1-Phe261
|
| 蛋白编码 |
E5BBQ3
|
| 种属 |
Thermobifida fusca
|
| 计算分子量 |
29.5 kDa
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| 表观分子量 |
28-30 kDa
|
| 标签 |
C-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM HAc-NaAc, 50% Glycerol, 5% Mannitol, 0.02% Tween 80, pH4.5.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Cutinase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is cutin hydrolase. Cutinase is a serine esterase containing the classical Ser, His, Asp triad of serine hydrolases. The protein belongs to the alpha-beta class, with a central beta-sheet of 5 parallel strands covered by 5 helices on either side of the sheet. Cutin monomers released from the cuticle by small amounts of cutinase on fungal spore surfaces can greatly increase the amount of cutinase secreted by the spore. The active site cleft is partly covered by 2 thin bridges formed by amino acid side chains, by contrast with the hydrophobic lid possessed by other lipases. The protein also contains 2 disulfide bridges, which are essential for activity, their cleavage resulting in complete loss of enzymatic activity.
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实验操作视频
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