TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit
中文名称:转化生长因子β1(TGF-β1)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗TGF-β1抗体包被于酶标板上,实验时样品(或标准品)中的TGF-β1会与包被抗体结合。后依次加入生物素化的抗TGF-β1抗体和辣根过氧化物酶标记的亲和素,抗TGF-β1抗体与结合在包被抗体上的TGF-β1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TGF-β1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TGF-β1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Universal |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的TGF-β1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Nanocurcumin preserves kidney function and haematology parameters in DMBA-induced ovarian cancer treated with cisplatin via its antioxidative and anti-inflammatory effect in rats
IF: 3.889Journal:PHARMACEUTICAL BIOLOGY -
Anti-inflammatory Mechanism of Geniposide: Inhibiting the Hyperpermeability of Fibroblast-Like Synoviocytes via the RhoA/p38MAPK/NF-κB/F-Actin Signal Pathway
IF: 3.831Journal:Frontiers in Pharmacology -
Polyherbal formulation: The studies towards identification of composition and their biological activities
IF: 3.829Journal:JOURNAL OF KING SAUD UNIVERSITY SCIENCE -
Induction of CD4+CD25+ Regulatory T Cells from In Vitro Grown Human Mononuclear Cells by Sparteine Sulfate and Harpagoside
IF: 3.796Journal:Biology-Basel -
I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis
IF: 3.758 -
Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
IF: 3.743 -
The osteoimmunomodulatory effect of nanostructured TiF x /TiO x coating on osteogenesis induction
IF: 3.715Journal:Biomedical Materials -
Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways
IF: 3.69 -
Polydatin combats methotrexate-induced pulmonary fibrosis in rats: Involvement of biochemical and histopathological assessment
IF: 3.652Journal:JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY -
Dimethyl fumarate ameliorates diabetes-associated vascular complications through ROS-TXNIP-NLRP3 inflammasome pathway
IF: 3.647Journal:LIFE SCIENCES