Human HMGB-1(High Mobility group protein B1) ELISA Kit
中文名称:人高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
别称:
HMGB1、HMG1、HMG3、SBP-1、Amphoterin
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人HMGB-1抗体包被于酶标板上,实验时样品(或标准品)中的人HMGB-1会与包被抗体结合。后依次加入生物素化的抗人HMGB-1抗体和辣根过氧化物酶标记的亲和素,抗人HMGB-1抗体与结合在包被抗体上的人HMGB-1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,HMGB-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人HMGB-1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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