Human HMGB-1(High Mobility group protein B1) ELISA Kit
中文名称:人高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
别称:
HMGB1、HMG1、HMG3、SBP-1、Amphoterin
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人HMGB-1抗体包被于酶标板上,实验时样品(或标准品)中的人HMGB-1会与包被抗体结合。后依次加入生物素化的抗人HMGB-1抗体和辣根过氧化物酶标记的亲和素,抗人HMGB-1抗体与结合在包被抗体上的人HMGB-1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,HMGB-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人HMGB-1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling
IF: 4.162 -
HMGB1-induced endothelial cell pyroptosis is involved in systemic inflammatory response syndrome following radiofrequency ablation of hepatic hemangiomas
IF: 4.06Journal:American Journal of Translational ResearchPMID:31934300 -
Response of Circulating Inflammatory Markers to Intermittent Hypoxia-Hyperoxia Training in Healthy Elderly People and Patients with Mild Cognitive Impairment
IF: 3.817Journal:Life-Basel -
Ethanol directly induced HMGB1 release through NOX2/NLRP1 inflammasome in neuronal cells
IF: 3.621Journal:TOXICOLOGY -
The Value of Extracellular Cold-Inducible RNA-Binding Protein (eCIRP) in Predicting the Severity and Prognosis of Patients After Cardiac Arrest: A Preliminary Observational Study
IF: 3.454 -
Short-Term Postoperative Cognitive Dysfunction and Inflammatory Response in Patients Undergoing Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study
IF: 3.232Journal:MEDIATORS OF INFLAMMATION -
Evidence for SIRT1 Mediated HMGB1 Release From Kidney Cells in the Early Stages of Hemorrhagic Shock
IF: 3.201Journal:Frontiers in Physiology -
The association between plasma HMGB1 and sRAGE and clinical outcome in intracerebral hemorrhage
IF: 3.125Journal:JOURNAL OF NEUROIMMUNOLOGY -
Role of toll-like receptor 4 in the regulation of the cell death pathway and neuroinflammation
IF: 3.103 -
29-kDa FN-f inhibited autophagy through modulating localization of HMGB1 in human articular chondrocytes
IF: 3.085Journal:BMB Reports