Human IL-8(Interleukin 8) ELISA Kit
中文名称:人白介素8(IL-8)酶联免疫吸附测定试剂盒
别称:
IL8、CXCL8、GCP-1、GCP1、LECT、LUCT、LYNAP、MDNCF、MONAP、NAF、NAP-1、NAP1、3-10C、AMCF-I、K60
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-8抗体包被于酶标板上,实验时样品(或标准品)中的人IL-8会与包被抗体结合。后依次加入生物素化的抗人IL-8抗体和辣根过氧化物酶标记的亲和素,抗人IL-8抗体与结合在包被抗体上的人IL-8结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-8浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-8的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IL-8,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output
IF: 38.637 -
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
IF: 11.556 -
Transient post-operative overexpression of CXCR2 on monocytes of traumatic brain injury patients drives monocyte chemotaxis toward cerebrospinal fluid and enhances monocyte-mediated immunogenic cell death of neurons in vitro
IF: 9.587 -
METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner
IF: 8.786 -
Decreased CXCR2 expression on circulating monocytes of colorectal cancer impairs recruitment and induces Re-education of tumor-associated macrophages
IF: 8.679 -
A novel mechanism for A-to-I RNA-edited AZIN1 in promoting tumor angiogenesis in colorectal cancer
IF: 8.469 -
Cathepsin F and Fibulin-1 as novel diagnostic biomarkers for brain metastasis of non-small cell lung cancer
IF: 7.64 -
EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma
IF: 6.684 -
Magnoflorine attenuates inflammatory responses in RA by regulating the PI3K/Akt/NF-κB and Keap1-Nrf2/HO-1 signalling pathways in vivo and in vitro
IF: 6.656 -
Salivary Interleukin Levels in Oral Squamous Cell Carcinoma and Oral Epithelial Dysplasia: Findings from a Sri Lankan Study
IF: 6.575Journal:Cancers
E-OSEL-H0014 | QuicKey Pro Human IL-8(Interleukin 8) ELISA Kit | 96T , 48T , 96T*5 |