Human IL-8(Interleukin 8) ELISA Kit
中文名称:人白介素8(IL-8)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-8抗体包被于酶标板上,实验时样品(或标准品)中的人IL-8会与包被抗体结合。后依次加入生物素化的抗人IL-8抗体和辣根过氧化物酶标记的亲和素,抗人IL-8抗体与结合在包被抗体上的人IL-8结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-8浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-8的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人IL-8,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells
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Grape Pomace for Topical Application: Green NaDES Sustainable Extraction, Skin Permeation Studies, Antioxidant and Anti-Inflammatory Activities Characterization in 3D Human Keratinocytes
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Surface-layer protein from Lactobacillus acidophilus NCFM attenuates tumor necrosis factor-α-induced intestinal barrier dysfunction and inflammation
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Downregulation of miRNA miR-1305 and upregulation of miRNA miR-6785-5p may be associated with psoriasis
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A Randomized Controlled Trial Evaluating the Levels of the Biomarkers hs-CRP, IL-6, and IL-8 in Patients with Temporomandibular Disorder Treated with LLLT, Traditional Conservative Treatment, and a Combination of Both
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ILF2 Contributes to Hyperproliferation of Keratinocytes and Skin Inflammation in a KLHDC7B-DT-Dependent Manner in Psoriasis
IF: 4.599Journal:Frontiers in Genetics
E-OSEL-H0014 | QuicKey Pro Human IL-8(Interleukin 8) ELISA Kit | 96T , 48T , 96T*5 |