Human IL-8(Interleukin 8) ELISA Kit
中文名称:人白介素8(IL-8)酶联免疫吸附测定试剂盒
别称:
IL8、CXCL8、GCP-1、GCP1、LECT、LUCT、LYNAP、MDNCF、MONAP、NAF、NAP-1、NAP1、3-10C、AMCF-I、K60
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-8抗体包被于酶标板上,实验时样品(或标准品)中的人IL-8会与包被抗体结合。后依次加入生物素化的抗人IL-8抗体和辣根过氧化物酶标记的亲和素,抗人IL-8抗体与结合在包被抗体上的人IL-8结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-8浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-8的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人IL-8,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Validation of Salivary Markers, IL-1β, IL-8 and Lgals3bp for Detection of Oral Squamous Cell Carcinoma in an Indian Population
IF: 3.998Journal:Scientific Reports -
Somatization is associated with altered serum levels of vitamin D, serotonin, and brain-derived neurotrophic factor in patients with predominant diarrhea irritable bowel syndrome
IF: 3.96Journal:NEUROGASTROENTEROLOGY AND MOTILITY -
Mammalian Ste20-like kinase 4 inhibits the inflammatory response in Aspergillus fumigatus keratitis
IF: 3.943 -
The liver X receptor agonist T0901317 reduces the inflammation of alveolar epithelial cells induced by polyhexamethylene guanidine through inhibition of the NFκB signaling pathway
IF: 3.932Journal:Annals of Translational Medicine -
Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF
IF: 3.932Journal:Annals of Translational Medicine -
MicroRNA-218 acts by repressing TNFR1-mediated activation of NF-κB, which is involved in MUC5AC hyper-production and inflammation in smoking-induced bronchiolitis of COPD
IF: 3.858Journal:TOXICOLOGY LETTERS -
Nec-1 Attenuates Neurotoxicity Induced by Titanium Dioxide Nanomaterials on Sh-Sy5y Cells Through RIP1
IF: 3.578Journal:Nanoscale Research Letters -
Roflumilast Reduced the IL-18-Induced Inflammatory Response in Fibroblast-Like Synoviocytes (FLS)
IF: 3.512 -
Association between pro-inflammatory cytokines in cerebrospinal fluid and headache in patients with aneurysmal subarachnoid hemorrhage
IF: 3.478 -
Eriodictyol inhibits survival and inflammatory responses and promotes apoptosis in rheumatoid arthritis fibroblast‐like synoviocytes through AKT/FOXO1 signaling
IF: 3.448Journal:JOURNAL OF CELLULAR BIOCHEMISTRY
E-OSEL-H0014 | QuicKey Pro Human IL-8(Interleukin 8) ELISA Kit | 96T , 48T , 96T*5 |