Human IL-8(Interleukin 8) ELISA Kit
中文名称：人白介素8(IL-8)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-8抗体包被于酶标板上，实验时样品（或标准品）中的人IL-8会与包被抗体结合。后依次加入生物素化的抗人IL-8抗体和辣根过氧化物酶标记的亲和素，抗人IL-8抗体与结合在包被抗体上的人IL-8结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-8浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-8的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆、细胞上清、细胞提取液体、组织匀浆等其他生物体液 |
| 特异性 | 可检测样本中的人IL-8，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃/-20℃ |
| 有效期 | 6个月 |
| 数据处理 |
-
Effect of Lactobacillus acidophilus Fermented Broths Enriched with Eruca sativa Seed Extracts on Intestinal Barrier and Inflammation in a Co-Culture System of an Enterohemorrhagic Escherichia coli and Human Intestinal Cells
IF: 4.546 -
Slit guidance ligand 2 promotes the inflammatory response of periodontitis through activation of the NF-κB signaling pathway
IF: 4.419 -
CircTHBS1 facilitates the progression of interstitial cystitis depending on the regulation of miR-139-5p/MFN2 axis
IF: 4.36 -
Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways
IF: 4.36 -
Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
IF: 4.3Journal：International Journal of Nanomedicine -
NRF2 -617 C/A Polymorphism Impacts Proinflammatory Cytokine Levels, Survival, and Transplant-Related Mortality After Hematopoietic Stem Cell Transplantation in Adult Patients Receiving Busulfan-Based Conditioning Regimens
IF: 4.225 -
Sodium Tanshinone II Sulfonate A Ameliorates Hypoxia-Induced Pulmonary Hypertension
IF: 4.225 -
Occupational exposure of rural workers to pesticides in a vegetable-producing region in Brazil
IF: 4.223 -
Lycium barbarum polysaccharide-glycoprotein promotes osteogenesis in hPDLSCs via ERK activation
IF: 4.068Journal：ORAL DISEASES -
The effects of Mediterranean diet on hepatic steatosis, oxidative stress, and inflammation in adolescents with non-alcoholic fatty liver disease: A randomized controlled trial
IF: 4
实验操作视频
